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![]() I1 and/or I2 FASTQ files are optional.įor more information on FASTQ format requirements, please see Specifying FASTQ files. Note that only R1 and R2 FASTQ files are required for Cell Ranger. compatible file name: SRR9291388_S1_R1_ (for Cell Ranger v4.0 and later)Ĭhanging the file names will allow Cell Ranger (version >=2.1.1) to accept these data as input.compatible file name: SRR9291388_S1_L001_R1_ Sequence Read Archive (SRA) data, available through multiple cloud providers and NCBI servers, is the largest publicly available repository of high throughput sequencing data.incompatible file name: SRR9291388_1.fastq.gz.NOTE: Cell Ranger v4.0 and later will accept file names without lane number. The number of FASTQ files we retrieved is consistent with what is reported in the metadata of SRR9291388: This run has 3 reads per spot.īased on the read length and the attributes listed in the metadata, we know that:Ĭell Ranger requires FASTQ file names to follow the bcl2fastq file naming convention. The output would be three FASTQ files: SRR9291388_1.fastq.gz For example, if we try to retrieve FASTQ files from SRR9291388: fastq-dump -split-files -gzip SRR9291388 Sometimes, the authors may upload 3 or 4 reads for a sample by including index reads. Download and convert SRA files to FASTQ files using the NCBI’s SRA toolkit. The number of FASTQ files we retrieved is consistent with what is reported in the metadata of SRR6334436: This run has 2 reads per spot.īased on the read length, we can infer that SRR6334436_1.fastq is Read 1 and SRR6334436_2.fastq is Read 2 (see Sequencing Recommendation for 3' Gene Expression Assays) The output would be two FASTQ files: SRR6334436_1.fastq.gz ![]() The command may look like this: fastq-dump -split-files -gzip SRR6334436 The following fix has been tested on Chromium v2 and v3 chemistry.įirst, use the NCBI fastq-dump utility with the -split-files argument to retrieve the FASTQ files. The prefetch - tool can be invoked multiple times if the download did not succeed. The prefetch tool downloads all necessary files to your computer. The combination of prefetch + fasterq-dump is the fastest way to extract FASTQ-files from SRA-accessions. Before downloading SRA data, first identify the platform and version of the chemistry used to generate the data. How to use prefetch and fasterq-dump to extract FASTQ-files from SRA run accessions. Using these data in Cell Ranger requires some pre-processing. The primary source of these publicly available datasets in the United States is the Sequence Read Archive (SRA) maintained by NCBI. ![]() Question: How do I prepare Sequence Read Archive (SRA) data from NCBI for Cell Ranger?Īnswer: One of the beauties of open source data in the sequencing age is the ability to reanalyze data generated by other researchers. However, that’s not the only ace up MEGA’s sleeves. This offer makes MEGA a handy choice for free users. You can increase your storage by completing certain actions, including installing MEGA’s apps, inviting friends, and verifying your mobile number. This is about ten times what Dropbox offers. With 20GB of free storage, MEGA is one of the best free Dropbox alternatives. Google stores a truckload of information about users, and it has suffered security breaches in the past. However, there are also privacy concerns with Google Drive. If you need more space, you can purchase a Google One subscription of up to 2TB of storage. ![]() Note that this storage is shared across all of Google’s apps, so you might burn through it quickly. Google Drive is a no-brainer if you already use Google’s products, including Gmail, Docs, Sheets, Sites, Calendar, and Keep.įurthermore, Google Drive offers a rather generous 15GB of monthly storage. First, it’s an excellent choice for team collaboration, thanks to its seamless integration with in-house Google and third-party applications. Google Drive is considered one of the best Dropbox alternatives for many reasons. Google Drive – Popular team collaboration tool Sync’s main downside is that it doesn’t have many third-party integrations. Many other cloud services offer much bigger free storage, but this is more than double what Dropbox offers. Even without subscribing to a paid plan, Sync gives you 5GB of free storage. These features make Sync a smart choice if you often send and receive sensitive files.įurthermore, Sync offers unlimited storage, which is ideal if you need to store, transfer and receive lots of files. Its end-to-end encryption feature ensures unauthorized persons can’t read or tamper with your data. Sync offers zero-knowledge encryption, which means that you’re the only one who has access to your encryption keys. When it comes to security and privacy, Sync is a far better choice than Dropbox. Sync – Great security and privacy features Next, we’ll discuss these best Dropbox alternatives in more detail. ** This service provider only offers team plans starting at 100 users. $6.99 (1TB) or sign up with Amazon Prime for unlimited storage Great for storing an unlimited number of photos Secure cloud storage service that’s great for businessesĪn excellent backup alternative to restore previous versions of filesĬloud storage service for storing photos and audio Unlimited storage (14-day free trial option)Ĭloud storage service with a modern and sleek interface Great for team collaboration with third-party integrations Great service for Windows and Microsoft Office usersĮncrypted cloud storage with many security and privacy features Generous free cloud storage service with top-notch file versioningĪ feature-rich alternative for backing up filesĪ cloud storage solution that offers lifetime plansĪ cloud backup service that offers unlimited secure storage ServiceĪ secure cloud storage solution with airtight securityĪn excellent choice for team collaboration and Google Workspace users So, we recommend opting for one of the Dropbox alternatives in the table below. The amount of storage offered to free accounts is also a problematic feature. ![]() Dropbox Alternativesĭropbox is one of the most popular tools for file sharing, but there are privacy issues with the service provider that have come to light in the past. In this article, we’ll show 20 cloud storage services that you can use instead of Dropbox. We’ve researched and tested dozens of cloud storage solutions. So, if you’re looking for the best Dropbox alternatives, we’ve done the hard work for you. It offers overpriced subscription plans. ![]()
![]() Your time is yours to do with as you choose, of course, and we respect that you’ll need plenty of time to dream, reflect and create. You’ll find that the Key West community is very welcoming to new people (especially creative ones!) – and there is never a shortage of things to do. They provided your conch cruiser or tricycle. WE*CYCLE.The fun way to get around town! Public transportation with 2 wheels. Lazy Dog does it all – kayak and paddle board tours, rentals, lessons and classes, paddle yoga and fun boat adventures! They’ve offered a paddle pass for PEARs – simply inquire with staff about an available day and they’ll hook you up with a free rental. Key West Library has an extensive catalog of books and other media, plus e-books and audiobooks available for download and even some special events! Their calendar has more about what’s going on. ![]() We’ve partnered with the library to offer you a library card to use during your stay. Zach Beach by 8:15am to flow with Nancy and Don. KEY WEST YOGA SANCTUARY.Free yoga classes all month! Register for your first class online with code ‘PEAR’ then connect with staff to get set up with a full month at your first visit. There’s one pass for the whole house, so ask us for it, or coordinate with your fellow PEARs on good days to share. We’ve got a State Pass for you to use during your stay. Generously provided your linens, bedding and towels.įORT ZACHARY TAYLOR STATE PARK. Through generous sponsors we are able to provide the following extras to use during your residency:īEST OF KEY WEST RENTALS. ![]() Those aspects aren’t a necessity when it comes to video editing, and I’ve worked on many projects without wrapping my head around them. You’ll also need to be savvy with the technical aspects of post-production, delivery formats, and the high-end production process. It requires you to understand color space, media management, project setups, and frame rates. On the other hand, DaVinci Resolve Studio caters more to the technical editing spectrum. ![]() Yet, with time, it grew big on editors as it saves you a considerable amount of time once you master its ins and outs. When it first emerged, some professional video editors weren’t big on the magnetic timeline, claiming that it resembled iMovie too much. It gives you drag-and-drop simplicity with a magnetic timeline. It’s safe to say that if you’re looking for creative editing software for Macs, Final Cut Pro is an excellent option.
Copilot will use existing layouts to build a presentation for you. Starting with a template will let Copilot know that you would like to retain the presentation’s theme and design. If your organization uses a standard template, start with this file before creating a presentation with Copilot. If you have images that you would like to be brought over to your presentation, be sure to include them in your Word document. When creating a presentation, Copilot will try to incorporate the images in your Word document. Include images that are relevant to your presentation Structure your content under Titles and Headers when appropriate and Copilot will do its best to generate a presentation for you. Paste the URL to your Word document into CopilotĬopilot will draft a presentation for you!Įdit the presentation to suit your needs, ask Copilot to add a slide, organize your presentation, or add images.īest practices when creating a presentation from a Word document Leverage Word Styles to help Copilot understand the structure of your documentīy using Styles in Word to organize your document, Copilot will better understand your document structure and how to break it up into slides of a presentation. ![]() Copy the link from the Share tray for best results! Tip: Copilot can occasionally have trouble using the URL from your address bar. Navigate to Word and copy the URL of the document you'd like to use by opening the share tray in Word and clicking Copy Link Tip: Currently, Copilot is only able to create presentations from Word documents. Select the Copilot button in the Home tab of the ribbon.Ĭlick or type Create presentation from file. Provide Copilot in PowerPoint with the link to your Word document, and it will generate slides, apply layouts, and choose a theme for you. With Copilot in PowerPoint, you can create a presentation from an existing Word document. Word Editor Online app is available around the clock and always at your fingertips.Create a presentation from a file with Copilot Take advantage of this lightweight alternative to heavy-duty office editors, and streamline your Word document editing process like never before. Experience the freedom of seamless accessibility, efficient editing capabilities, and the ability to save your Word files in the format of your choice. Create, Edit Word onlineĮmbrace the simplicity and versatility of our Online Word Editor, the ultimate solution for editing Word documents online. You can choose from a range of output file formats to ensure compatibility with other software and platforms. Once you've finished modifying your document, our free service offers a convenient way to download your edited file back to your device. To enhance your editing experience, we've implemented a full-screen mode that eliminates distractions and allows you to focus solely on the content of your Word file. With a look similar to traditional office editors and a standard set of tools, you'll quickly feel at home. Our user-friendly interface ensures a smooth and efficient workflow, allowing you to effortlessly modify text, format paragraphs, edit styles, and more. ![]() Edit Word file onlineĮditing a Word document has never been easier. ![]() With just a few clicks, you can start working on a Word file on any Internet-enabled device. Open, view and edit Word documents on the go. This handy Online Word Editor app makes it easy to create new documents or modify existing ones. As a lightweight alternative to powerful office editors, it provides a user-friendly interface, comprehensive formatting capabilities, and seamless file format management. 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It is especially useful for removing lead-based paint from windows, doors, and other areas where lead-based paint may be present. If you’re looking for an easy way to improve your shooting performance, give shooting a Lead Sled Without Weights a try! What is a Lead SledĪ Lead Sled is a tool that allows you to safely and effectively clean lead-based paint from surfaces. And finally, it minimizes muzzle rise so you can stay on target better between shots. Second, it’s more comfortable to shoot because there’s no added weight on the front of the gun. So why use a lead sled without weights? There are several reasons.įirst, it’s much easier to set up and take down than a traditional lead sled with weights. This means that when the rifle recoils, it will rotate around this point instead of moving back into your shoulder. When you tighten them down, they create a pivot point for the rifle. These arms are adjustable, so you can position them however you like. Here’s how it works: The lead sled actually has two arms that come down and rest on either side of the rifle’s forend. But what many shooters don’t realize is that you don’t need weights on the lead sled in order for it to work properly! A lead sled is a device that allows you to shoot without having to hold the rifle up, which can be helpful if you’re shooting long distances or trying to minimize recoil. If you’re a competitive shooter, chances are you’ve considered using a lead sled. One-shot zero with the Caldwell Lead Sled However, it can also be used as a compliment, indicating that despite its size and weight, the car in question is still a powerful machine. Today, the term is still used colloquially to describe any large and unwieldy car. Over time, this lead weight became synonymous with the entire car, hence the term “lead sled.” The expression is thought to come from the fact that these types of cars often had lead weights added to them in order to improve traction and stability. It is most commonly used to refer to heavy, old-fashioned cars that are difficult to drive and require a lot of effort to keep on the road. ![]() The term “lead sled” is a slang expression that originated in the United States in the early 20th century. Additionally, depending on how the gun is mounted on the sled, it can be more difficult to access the trigger, which can also affect accuracy. The added weight of the sled can make the rifle more difficult to handle, and if not properly secured, the gun can move around on the sled, affecting accuracy. While a lead sled may help to stabilize a rifle for more accurate shooting, there are potential negatives that should be considered as well. So next time you head out to the range, don’t forget your trusty bags of sand! Does a Lead Sled Affect Accuracy? Other than that, using sandbags to fill your lead sled is a quick and easy way to get started shooting without breaking the bank. This means that you may need to add more sand periodically to keep them full and effective. You don’t want them sliding out from under your rifle mid-shot!Īlso, be aware that over time, the sand can settle and compress inside the bags. First, make sure that the bags are secure and won’t move around while you are shooting. ![]() There are a few things to keep in mind when using this method though. Just fill up the bags with sand and put them in the appropriate place on the lead sled. That’s right, all you need is some sandbags and a little bit of sand to get started. So, what do you fill lead sled bags with? The answer is actually quite simple – sand! It also has the added benefit of absorbing some of the recoil from your rifle, making it easier and more comfortable for you to shoot. A lead sled is simply a device that helps hold your rifle steady while you are taking aim. When you are ready to go shooting, there are a few things you will need to have on hand. The dog is then placed in the sled and the lead is used to pull the sled around. The lead sled consists of a metal frame with a lead attached to it. It is also used to help keep dogs from getting lost while they are out in the field. Put your desired weight in the Lead SledĪ lead sled is a device that is used to help train dogs to become hunting dogs.They can also be useful for benchrest shooters who need to minimize movement during firing. Lead sleds are often used when shooting long range, as they help to reduce recoil and prevent muzzle rise. The shooter places the butt of the gun in the sling and adjusts the weight until the gun is stable. It consists of a weight (usually lead shot) on a platform that can be adjusted to fit the gun. ![]() A lead sled is a device used to steady a firearm while shooting. ![]() If there are details you want to include that don't fit, you can also change the template itself to better suit your needs. ![]() Once you've entered your information, you can tweak and adjust the content to fit better into the template. Related: 90 of the best CV buzzwords to make your CV stand out 4. Depending on the style and format of the CV, you may have more or less room for different sections and types of information. Using the text as a guide, you can enter your information to complete your CV including any keywords or buzzwords that apply to the role. ![]() Word templates provide pre-filled content in each section to give you a guideline for what you could include on your CV. Enter information into the provided sections on your template You may also want to create a list of your key skills and abilities that apply to the role, ready to add them to your CV. Ensuring you have accurate information about your dates for employment and education, your qualifications and the job titles you have held forms a good foundation for your CV. Gather all the information for your CVīefore replacing the template information on your chosen CV, gather all the details you want to include. Word provides free access to their templates, allowing you to download and customise different CVs to find which one best suits your requirements. Using the search bar on the 'New Page' tab, you can search for different styles and finishes of CV to suit your specific needs. Microsoft Word features a range of different online templates accessible directly within the program itself. Search for CV templates in Microsoft Word Here are the steps you could follow to create a CV in Microsoft Word: 1. If you want to create a CV quickly for a specific job application or plan to make a CV for the first time, using Word can provide the resources you require. Related: Why is a CV important? everything you need to know How to create Word doc CVĬreating a CV using Word is a quick and easy process, with various templates and designs available for you to complete a professional-looking, tailored CV. For example, your CV for a graphic designer job may be more creative and colourful than a CV you use to apply for a job as a lawyer. Tailoring your CV design to a specific position can be an effective way to customise your application beyond your cover letter, with a range of Word templates available to add interest without causing distraction. Many employers review dozens or hundreds of CVs for each role, making it essential to have your details highlighted and readily available. A well-designed CV can help highlight the correct information with proper formatting, making your information easy to understand. ![]() A CV is most effective when it includes all the information required in a curated and condensed form, making it easier for employers to learn all about you in as short a time as possible. When you apply for jobs, your CV provides potential employers with insight into the skills, experience and education you have that may make you a suitable choice for the role. Related: The ultimate guide to cv basics (with example) The importance of a high-quality CV Word templates retain their formatting and style in all Microsoft programs, Google docs and PDF forms by design, helping to establish a level of professionalism for your job applications. Many employers expect you to create a CV using a digital platform like Word, with standardised templates and information that makes it easy to decide if you're a suitable candidate for a particular job. 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